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Burial and ritual activities in the cave from the Copper Age to the Middle Bronze Age have been recognized with implications on possible settlement pattern schemes with the La Sassa cave as a “persistent place” in the prehistoric human landscape. In the frame of a region that was not previously recognized as tectonically active, the structural evidence is relevant for understanding the speleogenesis of the cave from the Late Pleistocene and its human occupation. Sixteen 14C ages on fauna and human bones and thousands of archaeological finds provided chronological constraints of faulting in the Late Pleistocene and possibly also after the Middle Bronze Age. Both structural and 3D survey highlighted a step-wise shape of the cave due to normal fault steps that allowed the localized formation of concretions also enveloping archaeological layers. Here, we present the results of a multidisciplinary method that allowed us to recognize, for the first time in this area, the interaction between Late Pleistocene to Anthropocene neotectonic and archaeological evolutionary stages of a cave of the Apennines (La Sassa cave), that encompass also its surroundings (Volsci Range and Pontina Plain). Caves are one of the most conservative environments on Earth, where archaeological, anthropological, climatic and tectonic data can be well-preserved.